Systematic Characterization of Brassica napus HIR Gene Family Reveals a Positive Role of BnHIR2.7 in Sclerotinia sclerotiorum Resistance

Hypersensitive-induced response protein (HIR) is a class of plant immune proteins that play pivotal roles in Sclerotinia sclerotiorum (Lib.) de Bary resistance. However, there has been no systematic investigation and identification HIR genes rapeseed (Brassica napus L.). Hence, we identified 50 BnHIR classified them into four groups. Subcellular localization prediction suggested are mainly localized the mitochondria. Cis-acting elements involved light diverse abiotic stress were found promoter regions BnHIR. The majority Groups 1/3/4 expressed most examined tissues, especially leaves siliques pericarp, while Group 2 not or had low expression all detected tissues. In case S. inoculation, strongly induced, homologous 1, which exhibited different patterns. Moreover, overexpression BnHIR2.7 Arabidopsis thaliana illustrated its prominent resistance to sclerotiorum. Our study provides insight evolutionary relationships family B. lays foundation for their napus.